SEM Liquid Flow Brochure | Hummingbird Scientific

Study dynamic processes in controlled liquid environments with in-situ liquid-phase SEM. Supports static or continuous flow, optional reagent mixing, and MEMS chips for heating, electrical biasing, and electrochemistry.

Technical Specs

1400 Series SEM

Number of Inlets

1 or 2 depending on model, single outlet

Biasing Contacts

4 on-chip

Tubing Type

User-replaceable microfluidic tubing

Delivery System

Variable speed pressure or flow-controlled liquid delivery system

Tip Type

Removable tip

Flow Type

Continuous flow or static liquid

EDS Compatible

Yes

SEM Compatibility

Custom integration for most models

Available For:

www.hummingbirdscientific.com ©Hummingbird Scientific 2026 -The specifications provided are subject to change without notice.

Key Features and Capabilities

Reproducible Screw-Free Liquid-Cell Assembly

Achieve reproducible liquid-cell assembly with self-aligning windows and a screw-free sealing design

Removable, Swappable Tip and Tubing

Simplify cleaning with removable and upgradeable holder tips that allow user access for full tubing replacement

Flexible Liquid Cell Operation

Image samples in continuous-flow or static liquid environments using a sealed microfluidic chip assembly

Multimodal Imaging

Correlate electrochemical, heating, and imaging data across SEM, TEM, and X-ray microscopy platforms

Optimized for EDS

Perform correlative spectroscopy and microscopy for detailed in-situ elemental analysis

Integrated Liquid Heating

Optional add on

Add temperature-controlled liquid-phase SEM capabilities with homogeneous heating concurrent with electrical biasing, precise temperature regulation, and near-drift-free in-situ imaging

Dual-Flow Liquid Mixing

Optional add on

Accelerate discovery with dual-flow liquid mixing for in-situ liquid phase SEM, capturing dynamic liquid-liquid reactions with greater control and flexibility

Biologic SP-200 Potentiostat

Optional add on

Expand the capabilities of the SEM Liquid Flow Sample Holder with the BioLogic SP-200 potentiostat for quantitative in-situ TEM electrochemistry

www.hummingbirdscientific.com ©Hummingbird Scientific 2026 -The specifications provided are subject to change without notice.

Built for Diverse Applications

Magnetic polarization of nanocrystals within bacterial cells

Bio/Soft Materials

Visualize biological and soft matter systems dynamics, including growth, interactions, and degradation.

Biomineralization

Self-Assembly Processes

Metal-Organic Framework Dynamics

Soft Matter Dynamics

Biomolecular Interactions

Illustration of battery material particles and spectroscopy

Battery Materials

Directly visualize battery cycling, ion transport, dendrite formation, interface evolution, and structural changes.

Charge-Discharge Cycling

Electrochemistry

Study electrodeposition, electrocatalysis, corrosion mechanisms, and electrochemical interface evolution.

Cyclic Voltammetry (CV)

Linear Sweep Voltammetry (LSV)

Chronoamperometry (CA)

Chronopotentiometry (CP)

Electrochemical Impedance (EIS)

Featured Research

Dynamic multi-detector liquid-phase SEM imaging of nanoparticles in solution

a) Schematic of LC-SEM setup with BSE, STEM, and EDX detectors. b) The electrons scattered from the liquid cell are collected in both forward and backward directions. The forward scattered electrons are collected by the STEM detector, and the backward scattered electrons are collected by the BSE detector. c) Cu₂ONPs imaged with the different electron detectors: SE, BSE, and ADF-TE from left to right. The BSE and ADF-TE images were acquired with 0.1 M KHCO₃ electrolyte inside the assembled liquid cell. Scale bar is 25 nm in the inset.

Reference: Aram Yoon, et al. Microscopy and Microanalysis (2021). DOI:10.1017/S1431927620024769

www.hummingbirdscientific.com ©Hummingbird Scientific 2026 -The specifications provided are subject to change without notice.

Selected Publications

Calvin Parkin, Tyler Mefford, Jongwoo Lim, Aram Yoon, Rui Serra-Maia, Pawan Kumar, See Wee Chee, William C. Chueh, Daan Hein Alsem, Norman Salmon, Eric A. Stach. “In-Situ Liquid-Electrochemical X-Ray and Electron Microscopy for Multi-Modal Energy Materials Characterization”. ECS Meeting Abstracts (2024)

DOI:

10.1149/MA2024-01462591mtgabs

Alejandra Londono-Calderon, Srikanth Nayak, Curtis L. Mosher, Surya K. Mallapragada, Tanya Prozorov. “New approach to electron microscopy imaging of gel nanocomposites in situ”. Micron (2019)

DOI:

10.1016/j.micron.2019.02.010

Alejandra Londono-Calderon, Wan Sun, Zengyi Shao, Daan Hein Alsem, Tanya Prozorov. “Correlative Microbially-Assisted Imaging of Cellulose Deconstruction with Electron Microscopy”. Microscopy and Microanalysis (2018)

DOI:

10.1017/S1431927618002404

D.A. Fischer, D.H. Alsem, B. Simon, T. Prozorov, N. Salmon. “Development of an Integrated Platform for Cross-Correlative Imaging of Biological Specimens in Liquid using Light and Electron Microscopies”. Microscopy and Microanalysis (2013)

DOI:

10.1017/S1431927613004376