Key publications, open-source tools, and research directions in TEM automation, 4D-STEM, tomography, and AI-assisted microscopy.

Software is now central to modern transmission electron microscopy. Today’s TEM and STEM workflows depend on automated acquisition, microscope scripting, detector synchronization, tomography reconstruction, 4D-STEM analysis, machine learning, and increasingly autonomous experimental control.

This publication library curates influential papers, software platforms, and research directions that have shaped the software ecosystem for modern electron microscopy.

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In-Situ TEM Software
10.1039/C3NR04035F
TEM
Quantitative in situ TEM tensile fatigue testing on nanocrystalline metallic ultrathin films
Ehsan Hosseinian, Olivier N. Pierron
Nanoscale
2013

The authors develop quantitative methods for tensile fatigue testing of nanocrystalline ultrathin metallic films within a TEM. Real-time observations connect cyclic loading behavior with microstructural evolution and failure processes.

Data Analysis Software
10.1109/ISBI.2012.6235807
TEM
A Bayesian view on cryo-EM structure determination
Sjors H. W. Scheres
2012 9th IEEE International Symposium on Biomedical Imaging (ISBI)
2012

This paper explains cryo-EM structure determination from a Bayesian statistical perspective. The framework provides principled methods for handling uncertainty and improving reconstruction accuracy.

Simulation Software
10.1007/s11227-010-0540-x
TEM
Cardiac simulation on multi-GPU platform
Venkata Krishna Nimmagadda, Ali Akoglu, Salim Hariri, Talal Moukabary
The Journal of Supercomputing
2012

The paper demonstrates cardiac electrophysiology simulations accelerated across multiple GPUs. The implementation achieves substantial performance gains for large-scale biomedical computations.

Open-Source TEM Software
10.1109/ICPPW.2012.9
X-ray
Chunked Extendible Dense Arrays for Scientific Data Storage
Gideon Nimako, E.J. Otoo, Daniel Ohene-Kwofie
2012 41st International Conference on Parallel Processing Workshops
2012

The study proposes chunked extendible dense arrays for scientific data storage. The approach improves scalability and performance when managing large volumes of scientific information.

Data Analysis Software
10.1107/S0108767312099825
X-ray
Computational crystallography
B. Winkler
Acta Crystallographica Section A Foundations of Crystallography
2012

This article reviews the field of computational crystallography and its role in modern structural science. Computational methods are highlighted as essential tools for interpreting crystallographic data and material behavior.

Data Analysis Software
10.1016/j.jsb.2011.12.017
TEM
Dynamo: A flexible, user-friendly development tool for subtomogram averaging of cryo-EM data in high-performance computing environments
Daniel Castaño-Díez, Mikhail Kudryashev, Marcel Arheit, Henning Stahlberg
Journal of Structural Biology
2012

Dynamo provides a flexible platform for subtomogram averaging in cryo-electron tomography studies. The software supports high-performance computing workflows and detailed macromolecular structure analysis.

Data Analysis Software
10.1017/S1431927612006897
TEM
EDS Spectrum Imaging with Fast Fourier Transforms
R. Gauvin, P. Michaud, H. Demers, M.L. Trudeau
Microscopy and Microanalysis
2012

The authors apply fast Fourier transform techniques to EDS spectrum imaging datasets. The methodology enhances signal interpretation and supports efficient extraction of chemical information.

Data Analysis Software
10.1038/nmeth.2019
SEM
Fiji: an open-source platform for biological-image analysis
Johannes Schindelin, Ignacio Arganda-Carreras, Erwin Frise, Verena Kaynig, Mark Longair, Tobias Pietzsch, Stephan Preibisch, Curtis Rueden, Stephan Saalfeld, Benjamin Schmid, Jean-Yves Tinevez, Daniel James White, Volker Hartenstein, Kevin Eliceiri, Pavel Tomancak, Albert Cardona
Nature Methods
2012

Fiji extends ImageJ with a comprehensive collection of plugins and tools for biological image analysis. The open-source platform simplifies workflow development and supports reproducible research in microscopy and imaging sciences.

In-Situ TEM Software
10.1149/MA2012-01/42/1561
TEM
In Situ Studies of Lithium-Ion battery Solid-Electrolyte Interphase Formation Using Nonlinear Coherent Vibrational Spectroscopy
Prabuddha Mukherjee, Alexei Lagutchev, Dana D. Dlott
ECS Meeting Abstracts
2012

The authors investigate formation of the solid-electrolyte interphase in lithium-ion batteries using nonlinear vibrational spectroscopy. The study improves understanding of interfacial processes that influence battery performance.

4D-STEM Software
10.1149/MA2012-01/5/107
TEM
In Situ TEM Lithiation of Silicon
Xiao-Hua Liu, Yang Liu, John P. Sullivan, Ting Zhu, Li Zhong, L. Q. Zhang, Scott Mao, Akihiro Kushima, Ju Li, S. T. Picraux, Jian Yu Huang
ECS Meeting Abstracts
2012

This study investigates the lithiation of silicon using in-situ TEM techniques. Real-time observations reveal structural transformations, volume expansion, and reaction mechanisms important for lithium-ion battery anode development.

Data Analysis Software
10.1038/nmeth.2089
SEM
NIH Image to ImageJ: 25 years of image analysis
Caroline A Schneider, Wayne S Rasband, Kevin W Eliceiri
Nature Methods
2012

This landmark article reviews the evolution of NIH Image into ImageJ over twenty-five years of development. The platform has become a widely adopted tool for scientific image processing, analysis, and visualization across numerous research disciplines.

Open-Source TEM Software
10.1557/mrs.2011.332
TEM
Spectroscopic imaging in electron microscopy
Stephen J. Pennycook, Christian Colliex
MRS Bulletin
2012

This article reviews spectroscopic imaging methods in electron microscopy. By combining structural and chemical signals, these techniques enable detailed investigation of material composition and functionality.

Data Analysis Software
10.1016/B978-0-08-097133-9.00003-4
X-ray
The Method of Total Scattering and Atomic Pair Distribution Function Analysis
Pergamon Materials Series
2012

This chapter reviews total scattering methods and atomic pair distribution function analysis. The approach enables structural characterization of crystalline and disordered materials at the atomic scale.

Data Analysis Software
10.1017/S1431927611000456
SEM
An Open-Source Engine for the Processing of Electron Backscatter Patterns: EBSD-Image
Philippe T. Pinard, Marin Lagacé, Pierre Hovington, Denis Thibault, Raynald Gauvin
Microscopy and Microanalysis
2011

EBSD-Image is an open-source engine for processing electron backscatter diffraction patterns. The framework gives users flexible control over pattern enhancement, analysis procedures, and indexing workflows.

Simulation Software
10.1007/978-3-642-25646-2_40
TEM
Fast Algorithm for Lunar Craters Simulation
Li Jian-Sheng, Liu Wei-min, Lan Chao-zhen, Mao Xiao-yan, Lu Zhan-wei
Advances in Automation and Robotics, Vol. 2
2011

The authors develop a computationally efficient algorithm for simulating lunar crater landscapes. The method generates realistic surface features while reducing processing requirements.

Open-Source TEM Software
10.1007/978-1-4419-7719-9_10
X-ray
Hierarchical Data Format 5 : HDF5
Sandeep Koranne
Handbook of Open Source Tools
2011

This chapter introduces HDF5, a widely used hierarchical data format for scientific computing. The format provides efficient storage, organization, and retrieval of large and complex datasets.

In-Situ TEM Software
10.1017/S1431927611010245
TEM
In Situ TEM Monitoring of Thermal Decomposition in CdTe and ZnTe Nanowires
K Davami, S Mir Shah Ghassemi, R Shabazian-Yassar, J Lee, M Meyyappan
Microscopy and Microanalysis
2011

This study uses in-situ TEM to monitor thermal decomposition processes in CdTe and ZnTe nanowires. Real-time observations reveal structural evolution and degradation mechanisms under elevated temperatures.

In-Situ TEM Software
10.1016/j.scriptamat.2011.05.009
TEM
In situ TEM study on crack propagation in nanoscale Au thin films
Hongtao Wang, Anmin Nie, Jiabin Liu, Peng Wang, Wei Yang, Bangdao Chen, Hongzhong Liu, Maosen Fu
Scripta Materialia
2011

This study examines crack propagation in nanoscale gold thin films using in-situ TEM. Direct observation of fracture processes improves understanding of reliability and failure in nanoscale structures.

Tomography Software
10.1002/9780470015902.a0023175
TEM
Structure Determination by Single Particle Cryo‐electron Tomography
Thomas Hrabe, Friedrich Förster
Encyclopedia of Life Sciences
2011

The article reviews single-particle cryo-electron tomography for determining macromolecular structures in native environments. Combining tomography with averaging enables detailed visualization of biological assemblies.

Data Analysis Software
10.1007/978-3-642-11432-8_6
X-ray
Synchrotron-Based X-Ray Spectromicroscopy of Organic Nanoparticles Complexing Actinides
Markus Plaschke, Jörg Rothe, Melissa A. Denecke
Actinide Nanoparticle Research
2011

The chapter explores synchrotron X-ray spectromicroscopy for studying actinide interactions with organic nanoparticles. Advanced imaging and spectroscopy techniques reveal nanoscale chemical behavior.

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