Key publications, open-source tools, and research directions in TEM automation, 4D-STEM, tomography, and AI-assisted microscopy.

Software is now central to modern transmission electron microscopy. Today’s TEM and STEM workflows depend on automated acquisition, microscope scripting, detector synchronization, tomography reconstruction, 4D-STEM analysis, machine learning, and increasingly autonomous experimental control.

This publication library curates influential papers, software platforms, and research directions that have shaped the software ecosystem for modern electron microscopy.

Filters
2001
2026
From
To
From
To
Reset All Filters
Data Analysis Software
10.1016/j.jsb.2018.07.003
TEM
Single particle reconstruction and validation using Bsoft for the map challenge
J. Bernard Heymann
Journal of Structural Biology
2018

This paper demonstrates the use of Bsoft for single-particle reconstruction and validation in a community benchmarking challenge. The workflow emphasizes map quality assessment and reproducible cryo-EM analysis.

Data Analysis Software
10.1101/356584
TEM
SPHIRE-crYOLO: A fast and accurate fully automated particle picker for cryo-EM
Thorsten Wagner, Felipe Merino, Markus Stabrin, Toshio Moriya, Claudia Antoni, Amir Apelbaum, Philine Hagel, Oleg Sitsel, Tobias Raisch, Daniel Prumbaum, Dennis Quentin, Daniel Roderer, Sebastian Tacke, Birte Siebolds, Evelyn Schubert, Tanvir R. Shaikh, Pascal Lill, Christos Gatsogiannis, Stefan Raunser
2018

SPHIRE-crYOLO delivers fast and accurate automated particle selection through deep neural network methods. The tool accelerates cryo-EM processing pipelines and minimizes the need for manual curation.

Microscope Automation Software
10.2172/1440910
TEM
Structural Studies of Amorphous Materials by Fluctuation Electron Microscopy
Michael M. J. Treacy, Argonne National Laboratory University of Oxford
DOE
2018

This work explores fluctuation electron microscopy as a method for probing medium-range order in amorphous materials. The technique uncovers structural features that are difficult to detect using conventional diffraction methods.

In-Situ TEM Software
10.1017/S1431927618010127
TEM
Thermal Stability of High Entropy Alloys during in Situ TEM Heating.
Elaf A. Anber, Andrew C Lang, Eric A. Lass, Pranav Kumar Suri, Daniel Scotto D'Antuono, Haoyan Diao, Peter K Liaw, Mitra L Taheri
Microscopy and Microanalysis
2018

This work investigates the thermal stability of high-entropy alloys through in-situ TEM heating experiments. The observations provide insight into microstructural evolution and phase stability at elevated temperatures.

Tomography Software
10.1016/j.egypro.2018.09.042
TEM
Towards in-situ TEM for Li-ion Battery Research
Laura Wheatcroft, Doğan Özkaya, James Cookson, Beverley J Inkson
Energy Procedia
2018

This paper reviews progress toward implementing in-situ TEM techniques for lithium-ion battery research. Direct observation of electrochemical processes helps advance understanding of battery performance and failure mechanisms.

Data Analysis Software
10.1017/S1431927617011448
SEM
Accurate Grain and Phase Boundary Location by Dictionary-based Indexing of Geological EBSD Data
Saransh Singh, Katharina Marquardt, Marc De Graef
Microscopy and Microanalysis
2017

This study applies dictionary-based indexing to accurately identify grain and phase boundaries in geological EBSD datasets. The methodology improves crystallographic mapping and boundary characterization in complex materials.

Data Analysis Software
10.1186/s40679-017-0042-5
TEM
Atomap: a new software tool for the automated analysis of atomic resolution images using two-dimensional Gaussian fitting
Magnus Nord, Per Erik Vullum, Ian MacLaren, Thomas Tybell, Randi Holmestad
Advanced Structural and Chemical Imaging
2017

Atomap offers automated extraction of atomic column positions from high-resolution STEM images. By applying two-dimensional Gaussian fitting, the software improves structural quantification and reduces manual interpretation effort.

Data Analysis Software
10.1109/TII.2016.2641472
SEM
Defect Detection in SEM Images of Nanofibrous Materials
Diego Carrera, Fabio Manganini, Giacomo Boracchi, Ettore Lanzarone
IEEE Transactions on Industrial Informatics
2017

This study introduces methods for detecting defects in SEM images of nanofibrous materials. Automated image analysis supports quality assessment and manufacturing control of nanoscale fiber systems.

Data Analysis Software
10.1149/2.1041706jes
TEM
Electrochemical Measurements in In Situ TEM Experiments
Eli Fahrenkrug, Daan Hein Alsem, Norman Salmon, Stephen Maldonado
Journal of The Electrochemical Society
2017

This work examines electrochemical measurement strategies for in-situ TEM experiments. The framework strengthens the connection between electrochemical data and nanoscale structural observations.

Data Analysis Software
10.1017/S1431927617001751
TEM
Electron Microscopy (Big and Small) Data Analysis With the Open Source Software Package HyperSpy
Francisco De La Pena, Tomas Ostasevicius, Vidar Tonaas Fauske, Pierre Burdet, Petras Jokubauskas, Magnus Nord, Mike Sarahan, Eric Prestat, Duncan N. Johnstone, Joshua Taillon, Jan Caron, Tom Furnival, Katherine E. MacArthur, Alberto Eljarrat, Stefano Mazzucco, Vadim Migunov, Thomas Aarholt, Michael Walls, Florian Winkler, Gael Donval, Ben Martineau, Andreas Garmannslund, Luiz-Fernando Zagonel, Ilya Iyengar
Microscopy and Microanalysis
2017

The authors showcase HyperSpy as a comprehensive environment for electron microscopy data analysis. The software facilitates exploration, statistical processing, and visualization of datasets ranging from simple spectra to large multidimensional acquisitions.

Simulation Software
10.1017/S143192761700174X
SEM
EMsoft: open source software for electron diffraction/image simulations
Saransh Singh, Farangis Ram, Marc De Graef
Microscopy and Microanalysis
2017

EMsoft provides open-source tools for simulating electron diffraction patterns and electron microscopy images. The software supports materials characterization and method development through physics-based modeling.

In-Situ TEM Software
10.1039/C7CC01103B
TEM
Iridacycles for hydrogenation and dehydrogenation reactions
Chao Wang, Jianliang Xiao
Chemical Communications
2017

This review explores iridacycle compounds as catalysts for hydrogenation and dehydrogenation reactions. The discussion highlights their versatility, reaction mechanisms, and potential applications in synthesis.

Data Analysis Software
10.1038/nmeth.4193
TEM
MotionCor2: anisotropic correction of beam-induced motion for improved cryo-electron microscopy
Shawn Q Zheng, Eugene Palovcak, Jean-Paul Armache, Kliment A Verba, Yifan Cheng, David A Agard
Nature Methods
2017

MotionCor2 corrects anisotropic beam-induced specimen motion in cryo-electron microscopy images. The software increases image sharpness and contributes to higher-resolution structural results.

Microscope Automation Software
10.1017/S1431927617009576
TEM
Nanobeam Scanning Diffraction for Orientation Mapping of Polymers
Karen C. Bustillo, Ouliana Panova, X. Chelsea Chen, Christopher J. Takacs, Jim Ciston, Colin Ophus, Nitash P. Balsara, Andrew M. Minor
Microscopy and Microanalysis
2017

The authors demonstrate nanobeam scanning diffraction as a tool for mapping polymer orientations at high spatial resolution. The approach reveals local crystallographic organization and improves characterization of complex polymer structures.

Data Analysis Software
10.1117/12.2272585
X-ray
PyXRF: Python-based X-ray fluorescence analysis package
Hanfei Yan, Wei Xu, Dantong Yu, Annie Heroux, Wah-Keat Lee, Li Li, Stuart Campbell, Yong Chu
X-Ray Nanoimaging: Instruments and Methods III
2017

PyXRF is a Python-based package for analyzing X-ray fluorescence datasets. The framework supports elemental mapping, spectral fitting, and automated processing of synchrotron XRF experiments.

Data Analysis Software
10.1038/s41598-017-17204-5
SEM
QuPath: Open source software for digital pathology image analysis
Peter Bankhead, Maurice B. Loughrey, José A. Fernández, Yvonne Dombrowski, Darragh G. McArt, Philip D. Dunne, Stephen McQuaid, Ronan T. Gray, Liam J. Murray, Helen G. Coleman, Jacqueline A. James, Manuel Salto-Tellez, Peter W. Hamilton
Scientific Reports
2017

QuPath is an open-source platform designed for digital pathology and whole-slide image analysis. It provides tools for biomarker quantification, tissue classification, and large-scale pathology research workflows.

Simulation Software
10.1063/1.4985443
TEM
Resistive switching in memristive electrochemical metallization devices
Sven Dirkmann, Thomas Mussenbrock
AIP Advances
2017

This study investigates resistive switching mechanisms in memristive electrochemical metallization devices using three-dimensional kinetic simulations. The model captures ion transport, electric-field effects, and thermal processes that govern device performance.

Data Analysis Software
10.1107/S2053273317082122
SEM
Software for processing high-data-rate MX in CIF and NeXus/HDF5
Herbert Jacob Bernstein
Acta Crystallographica Section A Foundations and Advances
2017

This work describes software designed to process high-data-rate macromolecular crystallography datasets stored in CIF and NeXus/HDF5 formats. The tools support efficient handling of large experimental data streams.

Data Analysis Software
10.1016/j.carbon.2017.08.075
X-ray
Synchrotron X-ray characterization of crack strain fields in polygranular graphite
S.M. Barhli, L. Saucedo-Mora, M.S.L. Jordan, A.F. Cinar, C. Reinhard, M. Mostafavi, T.J. Marrow
Carbon
2017

The authors use synchrotron X-ray methods to characterize crack-induced strain fields in polygranular graphite. The analysis provides insight into damage evolution and fracture mechanisms in engineering materials.

Data Analysis Software
10.1017/S1431927617001817
SEM
The ImageJ Ecosystem: An Open and Extensible Platform for Biomedical Image Analysis.
Curtis T. Rueden, Kevin W. Eliceiri
Microscopy and Microanalysis
2017

This article reviews the broader ImageJ ecosystem and its role as an extensible platform for biomedical image analysis. The ecosystem supports plugin development, interoperability, and community-driven innovation.

1
-
20
of
420