Key publications, open-source tools, and research directions in TEM automation, 4D-STEM, tomography, and AI-assisted microscopy.

Software is now central to modern transmission electron microscopy. Today’s TEM and STEM workflows depend on automated acquisition, microscope scripting, detector synchronization, tomography reconstruction, 4D-STEM analysis, machine learning, and increasingly autonomous experimental control.

This publication library curates influential papers, software platforms, and research directions that have shaped the software ecosystem for modern electron microscopy.

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Data Analysis Software
10.1107/S1600577521004951
X-ray
<i>PyPhase</i> – a Python package for X-ray phase imaging
Max Langer, Yuhe Zhang, Diogo Figueirinhas, Jean-Baptiste Forien, Kannara Mom, Claire Mouton, Rajmund Mokso, Pablo Villanueva-Perez
Journal of Synchrotron Radiation
2021

PyPhase is a Python package developed for X-ray phase imaging and phase-retrieval applications. The software provides accessible reconstruction methods that facilitate broader adoption of phase-contrast imaging techniques.

In-Situ TEM Software
10.1017/S1431927621001914
TEM
Operando and in situ in a TEM imaging in a cryogenic temperature range
Martial Duchamp, Joseph Vas, Reinis Ignatans, Aaron David Mueller, Rohit Medwal, Rajdeep Rawat, Vasiliki Tileli
Microscopy and Microanalysis
2021

This study explores operando and in-situ TEM imaging at cryogenic temperatures. The platform enables direct observation of material behavior and dynamic processes under low-temperature conditions.

4D-STEM Software
10.22443/rms.mmc2021.200
TEM
Phase Object Reconstruction of 4D-STEM datasets using Deep Learning
Thomas Friedrich
Proceedings of the Microscience Microscopy Congress 2021 incorporating EMAG 2021
2021

This work applies deep learning techniques to reconstruct phase objects from 4D-STEM measurements. The approach extracts quantitative structural information from diffraction datasets while improving reconstruction efficiency and robustness.

Simulation Software
10.1016/j.micron.2021.103141
TEM
Prismatic 2.0 – Simulation software for scanning and high resolution transmission electron microscopy (STEM and HRTEM)
Luis Rangel DaCosta, Hamish G. Brown, Philipp M. Pelz, Alexander Rakowski, Natolya Barber, Peter O’Donovan, Patrick McBean, Lewys Jones, Jim Ciston, M.C. Scott, Colin Ophus
Micron
2021

Prismatic 2.0 delivers GPU-enabled simulations for STEM and HRTEM applications. The software accelerates image generation and allows researchers to investigate complex electron-specimen interactions efficiently.

Microscope Automation Software
10.1017/S1551929521000481
TEM
Probelab ReImager: An Open-Source Software for Streamlining Image Processing in an Electron Microscopy Laboratory
Noah Kraft, Anette Von Der Handt
Microscopy Today
2021

Probelab ReImager is an open-source application designed to streamline image processing in electron microscopy laboratories. The software simplifies preparation of publication-quality figures and analytical outputs.

Microscope Automation Software
10.1038/s41592-021-01087-6
TEM
Pycro-Manager: open-source software for customized and reproducible microscope control
Henry Pinkard, Nico Stuurman, Ivan E. Ivanov, Nicholas M. Anthony, Wei Ouyang, Bin Li, Bin Yang, Mark A. Tsuchida, Bryant Chhun, Grace Zhang, Ryan Mei, Michael Anderson, Douglas P. Shepherd, Ian Hunt-Isaak, Raymond L. Dunn, Wiebke Jahr, Saul Kato, Loïc A. Royer, Jay R. Thiagarajah, Kevin W. Eliceiri, Emma Lundberg, Shalin B. Mehta, Laura Waller
Nature Methods
2021

Pycro-Manager provides an open-source platform for customizable and reproducible microscope control. The software integrates automation, scripting, and data acquisition within flexible experimental workflows.

Simulation Software
10.1016/j.ejmp.2021.07.014
TEM
Radiomics software for breast imaging optimization and simulation studies
Stoyko Marinov, Ivan Buliev, Lesley Cockmartin, Hilde Bosmans, Zhivko Bliznakov, Giovanni Mettivier, Paolo Russo, Kristina Bliznakova
Physica Medica
2021

The paper presents radiomics software designed for breast imaging optimization and simulation research. The platform facilitates image quality assessment and evaluation of diagnostic workflow improvements.

Data Analysis Software
10.46608/primaluna8.9782356134073.8
SEM
SEM-EDS Elemental analysis of the pigments from Nauportus
Tina Berden, Adrijan Košir
Adriatlas 4. Produzioni artigianali in area adriatica: manufatti, ateliers e attori (III sec. a.C. – V sec. d.C.)
2021

The authors use SEM-EDS to analyze pigments recovered from the archaeological site of Nauportus. Elemental characterization provides information about historical materials and manufacturing practices.

Data Analysis Software
10.14513/actatechjaur.00573
SEM
Speckle pattern optimization for DIC technologies
Szabolcs Szalai, Gábor Dogossy
Acta Technica Jaurinensis
2021

The paper investigates optimization of speckle patterns for digital image correlation applications. Improved pattern design enhances measurement accuracy and reliability in experimental mechanics.

Data Analysis Software
10.1017/S1431927621004049
TEM
Strategies for Multimodal Analysis of Joint EELS and EDS Data
Ray Twesten, Jens Rafaelsen, Liam Spillane, Paul Thomas
Microscopy and Microanalysis
2021

The authors outline strategies for combining EELS and EDS measurements within unified analytical workflows. The approach enhances compositional interpretation by leveraging complementary spectroscopic information sources.

Data Analysis Software
10.1007/978-3-030-66329-2_3
X-ray
Synchrotron Beamlines, Instrumentation and Contributions
Marie-Christine Zdora
X-ray Phase-Contrast Imaging Using Near-Field Speckles
2021

This chapter reviews synchrotron beamlines, instrumentation, and their contributions to advanced imaging research. The discussion highlights the capabilities of modern synchrotron facilities in supporting cutting-edge scientific investigations.

Data Analysis Software
10.1038/s41598-020-79637-9
SEM
TEM, SEM, and STEM-based immuno-CLEM workflows offer complementary advantages
Viola Oorschot, Benjamin W. Lindsey, Jan Kaslin, Georg Ramm
Scientific Reports
2021

This paper discusses TEM-, SEM-, and STEM-based immuno-CLEM workflows and their complementary advantages. The methods integrate molecular labeling with ultrastructural imaging to improve biological tissue characterization.

Simulation Software
10.12688/openreseurope.13015.1
TEM
The abTEM code: transmission electron microscopy from first principles
Jacob Madsen, Toma Susi
Open Research Europe
2021

This article presents abTEM, a simulation package designed to model TEM imaging and diffraction from atomic structures. The framework bridges atomistic calculations and experimental observations through flexible first-principles-inspired workflows.

AI and Autonomous Microscopy Software
10.1007/978-3-030-75549-2_39
TEM
Total Deep Variation for Noisy Exit Wave Reconstruction in Transmission Electron Microscopy
Thomas Pinetz, Erich Kobler, Christian Doberstein, Benjamin Berkels, Alexander Effland
Scale Space and Variational Methods in Computer Vision
2021

This work proposes a total deep variation approach for reconstructing electron exit waves from noisy microscopy measurements. The method combines deep learning with variational optimization to improve image fidelity and recover fine structural details.

Data Analysis Software
10.1002/cphc.202000838
TEM
Vacancy assisted diffusion on single‐atom surface alloys
David Mahlberg, Axel Groß
ChemPhysChem
2021

This work investigates vacancy-assisted diffusion mechanisms in single-atom surface alloys. The findings provide insight into atomic mobility, surface stability, and catalytic behavior under operating conditions.

Tomography Software
10.1080/08940886.2021.1968243
X-ray
X-ray Computed Tomography at Synchrotron Facilities: Pipelines for Data Acquisition and Reduction
Lucia Mancini, Dula Parkinson, Alexander Rack, Christoph Rau, Francesco De Carlo
Synchrotron Radiation News
2021

The paper reviews data-acquisition and reduction pipelines used for X-ray computed tomography at synchrotron facilities. It highlights workflow optimization, automation, and efficient management of large imaging datasets.

Simulation Software
10.1017/S1431927620014701
TEM
abTEM: <i>ab Initio</i> Transmission Electron Microscopy Image Simulation
Jacob Madsen, Toma Susi
Microscopy and Microanalysis
2020

This contribution describes abTEM as a platform for generating realistic electron microscopy image simulations. The software supports atomistic modelling and helps researchers compare theoretical predictions with measured results.

Simulation Software
10.1017/S1431927620023326
TEM
A Python Based Open-source Multislice Simulation Package for Transmission Electron Microscopy
Hamish Brown, Phillipp Pelz, Colin Ophus, Jim Ciston
Microscopy and Microanalysis
2020

The authors introduce a Python-based multislice simulation package for transmission electron microscopy. The project emphasizes openness, flexibility, and accessibility while providing accurate electron scattering calculations.

In-Situ TEM Software
10.1017/S1431927620018048
TEM
Cryo-TEM Study of Interfaces in Rechargeable Lithium Batteries
Vahid Jabbari, Ramasubramonian Deivanayagam, Reza Shahbazian-Yassar
Microscopy and Microanalysis
2020

The authors use cryo-TEM to investigate interfacial structures in rechargeable lithium batteries. The observations provide valuable insight into degradation mechanisms and electrochemical performance.

Microscope Automation Software
10.1051/epjconf/202024501026
TEM
DAQling: an open-source data acquisition framework
Marco Boretto, Wojciech Brylinski, Giovanna Lehmann Miotto, Enrico Gamberini, Roland Sipos, Viktor Vilhelm Sonesten
EPJ Web of Conferences
2020

DAQling introduces a modular data acquisition framework designed around reusable software components. The system simplifies development and deployment of scalable data-collection infrastructures.

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