Key publications, open-source tools, and research directions in TEM automation, 4D-STEM, tomography, and AI-assisted microscopy.

Software is now central to modern transmission electron microscopy. Today’s TEM and STEM workflows depend on automated acquisition, microscope scripting, detector synchronization, tomography reconstruction, 4D-STEM analysis, machine learning, and increasingly autonomous experimental control.

This publication library curates influential papers, software platforms, and research directions that have shaped the software ecosystem for modern electron microscopy.

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Data Analysis Software
10.1051/matecconf/202235501004
SEM
Deformation mechanism of neutron irradiated Al-B based on SEM-DIC
Jiaqi Xiang, Qijie Feng, Junchao Cheng, Lei Lu, Junyu Huang, Zhengye Zhong
MATEC Web of Conferences
2022

This research examines deformation mechanisms in neutron-irradiated aluminum-boron materials using SEM-based digital image correlation. Full-field strain measurements reveal the impact of irradiation-induced microstructural changes.

Data Analysis Software
10.1002/9781119645719.ch8
X-ray
Elemental Analysis Using Synchrotron Radiation X‐Ray Fluorescence
M. K. Tiwari
X‐Ray Fluorescence in Biological Sciences
2022

This chapter discusses elemental analysis using synchrotron-radiation X-ray fluorescence. High sensitivity and spatial resolution make the method valuable for biological and materials research.

Data Analysis Software
10.1098/rsta.2021.0300
X-ray
FAIR data pipeline: provenance-driven data management for traceable scientific workflows
Sonia Natalie Mitchell, Andrew Lahiff, Nathan Cummings, Jonathan Hollocombe, Bram Boskamp, Ryan Field, Dennis Reddyhoff, Kristian Zarebski, Antony Wilson, Bruno Viola, Martin Burke, Blair Archibald, Paul Bessell, Richard Blackwell, Lisa A. Boden, Alys Brett, Sam Brett, Ruth Dundas, Jessica Enright, Alejandra N. Gonzalez-Beltran, Claire Harris, Ian Hinder, Christopher David Hughes, Martin Knight, Vino Mano, Ciaran McMonagle, Dominic Mellor, Sibylle Mohr, Glenn Marion, Louise Matthews, Iain J. McKendrick, Christopher Mark Pooley, Thibaud Porphyre, Aaron Reeves, Edward Townsend, Robert Turner, Jeremy Walton, Richard Reeve
Philosophical Transactions of the Royal Society A: Mathematical, Physical and Engineering Sciences
2022

This paper presents the FAIR Data Pipeline, a provenance-driven framework for managing scientific data and workflows. The approach improves traceability, reproducibility, and transparency in data-intensive research environments.

4D-STEM Software
10.1016/j.ultramic.2022.113517
TEM
Free, flexible and fast: Orientation mapping using the multi-core and GPU-accelerated template matching capabilities in the Python-based open source 4D-STEM analysis toolbox Pyxem
Niels Cautaerts, Phillip Crout, Håkon W. Ånes, Eric Prestat, Jiwon Jeong, Gerhard Dehm, Christian H. Liebscher
Ultramicroscopy
2022

This study introduces accelerated orientation mapping workflows in Pyxem using multi-core CPUs and GPUs. The implementation speeds up template matching for large 4D-STEM datasets while maintaining accuracy for crystallographic characterization.

Tomography Software
10.1017/S1431927622003804
TEM
High Angle Liquid Cell TEM Tomography for In Situ Observation and 3D Reconstruction in Liquid
Partha Pratim Das, Jennifer Cookman, Alejandro Gómez Pérez, Sergi Plana-Ruiz, Miguel López-Haro, José J Calvino, Jesús Gonzalez Casablanca, Evangelos Grivas, Georgios Se Antipas, Nikolaos A Ntallis, Stavros Nicolopoulos
Microscopy and Microanalysis
2022

The study presents high-angle liquid-cell TEM tomography for three-dimensional reconstruction of materials in liquid environments. The method enables in-situ visualization of structures and processes with enhanced volumetric detail.

4D-STEM Software
10.1007/978-3-031-23220-6_15
TEM
Interactive, Cloud-Native Workflows on HPC Using KNoC
Evangelos Maliaroudakis, Antony Chazapis, Alexandros Kanterakis, Manolis Marazakis, Angelos Bilas
High Performance Computing. ISC High Performance 2022 International Workshops
2022

KNoC delivers cloud-native workflow capabilities for high-performance computing environments. The platform simplifies interactive application deployment and improves accessibility to distributed computing resources.

Tomography Software
10.1107/S160057752200282X
X-ray
<i>Tofu</i> : a fast, versatile and user-friendly image processing toolkit for computed tomography
Tomáš Faragó, Sergey Gasilov, Iain Emslie, Marcus Zuber, Lukas Helfen, Matthias Vogelgesang, Tilo Baumbach
Journal of Synchrotron Radiation
2022

Tofu is a user-friendly toolkit for computed-tomography image processing and reconstruction. Workflow-based design enables efficient handling of large imaging datasets and complex processing tasks.

AI and Autonomous Microscopy Software
10.1038/s43586-022-00107-9
TEM
Machine learning in scanning transmission electron microscopy
Nature Reviews Methods Primers
2022

This review summarizes the growing role of machine learning in scanning transmission electron microscopy. The field leverages artificial intelligence for image interpretation, automation, and materials discovery.

Data Analysis Software
10.1016/j.cageo.2021.104952
SEM
MudrockNet: Semantic segmentation of mudrock SEM images through deep learning
Abhishek Bihani, Hugh Daigle, Javier E. Santos, Christopher Landry, Maša Prodanović, Kitty Milliken
Computers & Geosciences
2022

MudrockNet uses deep learning for semantic segmentation of mudrock scanning electron microscopy images. The framework enables automated identification of geological features within complex microstructures.

Data Analysis Software
10.3390/nano12111818
SEM
Nanoparticle Detection on SEM Images Using a Neural Network and Semi-Synthetic Training Data
Jorge López Gutiérrez, Itzel Abundez Barrera, Nayely Torres Gómez
Nanomaterials
2022

This study applies neural networks trained with semi-synthetic datasets to detect nanoparticles in scanning electron microscopy images. The approach automates particle identification and measurement while reducing manual annotation effort.

Open-Source TEM Software
10.1017/S1431927622006109
TEM
Object Segmentation on Cryo-electron Tomography Data
Lan Dang, Steven J Ludtke
Microscopy and Microanalysis
2022

The authors explore object segmentation techniques for cryo-electron tomography datasets. The approach helps identify biological structures within noisy volumetric data and supports automated analysis workflows.

4D-STEM Software
10.2172/1847929
TEM
Open Reproducible Electron Microscopy Data Analysis
Christopher Harris
DOE
2022

This publication promotes transparent and reproducible practices for electron microscopy data analysis. The framework emphasizes open workflows, shareable tools, and reliable scientific results.

Data Analysis Software
10.3390/catal12020135
SEM
Particle Recognition on Transmission Electron Microscopy Images Using Computer Vision and Deep Learning for Catalytic Applications
Anna V. Nartova, Mikhail Yu. Mashukov, Ruslan R. Astakhov, Vitalii Yu. Kudinov, Andrey V. Matveev, Alexey G. Okunev
Catalysts
2022

The authors combine computer vision and deep learning techniques to recognize nanoparticles in transmission electron microscopy images. The workflow supports automated characterization of catalytic materials and particle populations.

In-Situ TEM Software
10.1017/S1431927622003683
TEM
Probing Structural Changes in Nanoparticles During CO-Oxidation Reaction via in situ TEM
Utkur Mirsaidov
Microscopy and Microanalysis
2022

The study uses in-situ TEM to monitor structural changes in nanoparticles during carbon monoxide oxidation reactions. The observations connect catalytic activity with dynamic morphological transformations.

Data Analysis Software
10.1007/s12350-020-02340-9
TEM
Quantification of amyloid deposition using bone scan agents
Takashi Kudo, Aiganym Imakhanova
Journal of Nuclear Cardiology
2022

This article discusses approaches for quantifying amyloid deposition using nuclear imaging agents originally developed for bone scans. The methodology supports assessment of disease burden and diagnostic evaluation.

Data Analysis Software
10.1111/jmi.13099
SEM
Simple automation of SEM‐EDS spectral maps analysis with Python and the edxia framework
Fabien Georget, William Wilson, Karen L. Scrivener
Journal of Microscopy
2022

This article describes Python-based automation of SEM-EDS spectral map analysis using the edxia framework. The workflow reduces manual effort and improves reproducibility in microstructural investigations.

Simulation Software
10.1088/2399-6528/ac6ab0
X-ray
Simulation of Bragg coherent diffraction imaging
A H Mokhtar, D Serban, M C Newton
Journal of Physics Communications
2022

The work presents simulation techniques for Bragg coherent diffraction imaging. Modeling diffraction patterns helps researchers interpret crystal defects, lattice distortions, and nanoscale structural features.

In-Situ TEM Software
10.1093/bioinformatics/btac365
TEM
STracking: a free and open-source Python library for particle tracking and analysis
Sylvain Prigent, Cesar Augusto Valades-Cruz, Ludovic Leconte, Jean Salamero, Charles Kervrann
Bioinformatics
2022

STracking provides a free and open-source Python library for particle tracking and motion analysis. The framework supports construction of reproducible pipelines for studying dynamic biological systems.

Tomography Software
10.17504/protocols.io.81wgb6qqqlpk/v1
TEM
Subtomogram averaging and classification v1
Shujun Cai, Pietro De Camilli
2022

This protocol outlines procedures for subtomogram averaging and classification of macromolecular structures within cryotomograms. The workflow enhances signal quality and supports higher-resolution structural interpretation.

Data Analysis Software
10.1017/S1431927622011643
TEM
Tomviz: An Open-Source Platform for Electron Tomography
Jonathan Schwartz, Chris Harris, Jacob Pietryga, Jonathan Rowell, Brianna Major, Patrick Avery, Utkarsh Ayachit, Berk Geveci, Alessandro Genova, Cory Quammen, Peter Ercius, Yi Jiang, Richard Robinson, Marcus D Hanwell, Robert Hovden
Microscopy and Microanalysis
2022

Tomviz combines visualization, reconstruction, and analysis capabilities in a single open-source platform for electron tomography. The environment promotes interactive exploration of volumetric datasets and reproducible processing pipelines.

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